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Chicken anti-Rabbit IgG (H&L), DyLight® 594 conjugated

Product Specifications

Background

Chicken anti-Rabbit IgG (H&L), DyLight®594 Conjugate is a secondary antibody conjugated to DyLight® 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L) .DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

CAS Number

9007-83-4

HS Code

38221900

Host

Chicken

Immunogen

Purified rabbit IgG, whole molecule

Clonality

Polyclonal

Field of Research

Anti-Rabbit

Dilution

1 : 20-1 : 2000 for most applications

Purity

Immunogen affinity purified chicken IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Precautions

Based on  immunoelectrophoresis, this antibody reacts with:  heavy (γ) chains on rabbit IgG  light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins.

Additionnal Information

Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative

Storage Conditions

Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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