Donkey anti-Goat IgG (H&L), DyLight® 594 conjugated

Donkey anti-Goat IgG (H&L), DyLight®594 Conjugate is a secondary antibody conjugated to DyLight® 594, which binds to all goat IgGs in immunological assays. DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Based on  immunoelectrophoresis, this antibody reacts with:   heavy chains on goat IgG   light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative

Ainla et al. (2013) . Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.

Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.