Anti-ABI1 | Abscisic acid insensitive 1
Product Specifications
Background
ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway. It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. Expressed in seeds and seedlings. Confined to lateral root caps and columella cells in roots. Induced by low temperature, drought, high salt, ABA and ethylene. Activates/represses SnRK2.6/SRK2E/OST1 in response to ABA-dependent stimuli. Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
828714
UniProt
P49597
Host
Rabbit
Reactivity
Arabidopsis thaliana
Not reactive in
Sorghum bicolor
Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080. Chosen peptide is not present in AtABI2.
Clonality
Polyclonal
Applications
Immunoprecipitation (IP), Western blot (WB)
Field of Research
Biosynthesis/regulation
Dilution
5 µg (IP for a 200 ul of a cell extract), 3 µg (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
47.5 kDa
Precautions
Important note: blocking with more than 3% skimmed milk will result in lack of signal for this antibody
Additionnal Information
It is of crucial importance to chose a material in which ABI1 protein is highly expressed like seeds or senescent leaf. This protein could not be detected using this antibody in plants grown under optimal (non stressed) conditions, The antibody detects both, recombinant and endogenous ABI1 proteins.
References & Citations
Mitula et al. (2015) . Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56 (12) :2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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