Anti-NPR1 | Nonexpresser of PR genes 1

NPR1 (regulatory protein NPR1) is a key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Controls the onset of systemic acquired resistance (SAR) . Subcellular localization: cytoplasm, nucleaus (following induction by salicylic acid treatment or after pathogen infection. Alternative names: BTB/POZ domain-containing protein NPR1, Non-inducible immunity protein 1, Nim1, Salicylic acid insensitive 1, Sai1, ATNPR1.

Please note that depending upon detection system you are using, longer exposure time may be required with this antibody. Chemiluminescent detection reagent in extreme femtogram range is necessary.

For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75°C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.

Li et al. (2025) . Salicylic acid and ROS signaling modulate hypocotyl elongation in darkness via NPR1 and EX1. Sci Adv. 2025 Oct 31;11 (44) :eadx4417. doi: 10.1126/sciadv.adx4417. Yao et al. (2023) . High air humidity dampens salicylic acid pathway and NPR1 function to promote plant disease.EMBO J. 2023 Nov 2;42 (21) :e113499.doi: 10.15252/embj.2023113499. Arenas-Alfonseca et al. (2021) Arabidopsis beta-cyanoalanine synthase mutation overcomes NADPH oxidase action in response to pathogens. J Exp Bot. 2021 Mar 26:erab137. doi: 10.1093/jxb/erab137. Epub ahead of print. PMID: 33770168.Nomoto et al. (2021) Suppression of MYC transcription activators by the immune cofactor NPR1 fine-tunes plant immune responses. Cell Rep. 2021 Dec 14;37 (11) :110125. doi: 10.1016/j.celrep.2021.110125. PMID: 34910911.Lei et al. (2020) . Construction of gold-siRNANPR1 nanoparticles for effective and quick silencing of NPR1 in Arabidopsis thaliana. DOI: 10.1039/D0RA02156C (Paper) RSC Adv., 2020, 10, 19300-19308

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.