Anti-V-PPase | vacuolar H+-pyrophosphatase

Product Specifications

Background

V-PPase (pyrophosphate-energized vacuolar membrane proton pump 1), EC=3.6.1.1, acts to establish proton gradient of often higher magnitude than H+-ATPase. Is involved in auxin transport and NaCl and drought tolerance. Alternative names: pyrophosphate-energized inorganic pyrophosphatase 1, H (+) -PPase 1, vacuolar proton pyrophosphatase 1, vacuolar proton pyrophosphatase 3

CAS Number

9007-83-4

HS Code

38221900

Gene ID

838138

UniProt

P31414

Host

Rabbit

Reactivity

Arabidopsis thaliana, Cucumis sativus, Dunaliella bardawil, Hordeum vulgare, Nicotiana tabacum, Picea abies, Vitis vinifera

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase, UniProt P31414, TAIR AT1G15690

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Vacuolar membrane

Dilution

1 : 8000 (ELISA), 1 : 100 (IL), 1 : 2000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

80.8 | 73 kDa (Arabidopsis thaliana)

Precautions

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel

Additionnal Information

Antibodies will detect target protein in 1 µg of a crude membrane preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, less than µg load per well should be sufficient

References & Citations

Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel) . 2022 Apr 25;11 (5) :836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Prinsi et al. (2020) . Root Proteomic Analysis of Two Grapevine Rootstock Genotypes Showing Different Susceptibility to Salt Stress. Int J Mol Sci. 2020 Feb 6;21 (3) . pii: E1076. doi: 10.3390/ijms21031076.Patir-Nebioglu et al. (2019) . Pyrophosphate modulates plant stress responses via SUMOylation. Elife. 2019 Feb 20;8. pii: e44213. doi: 10.7554/eLife.44213.Migocka et al. (2015) . Cucumber Metal Transport Protein CsMTP9 is a plasma membrane H+ -coupled antiporter involved in the Mn2+ and Cd2+ efflux from root cells. Plant J. 2015 Oct 20. doi: 10.1111/tpj.13056.Migocka et al. (2014) . Molecular and biochemical properties of two P 1B2 -ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2014 Sep 11. doi: 10.1111/pce.12447.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.