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Goat anti-Rabbit IgG (H&L), DyLight® 633 conjugated

Product Specifications

Background

Goat anti-rabbit IgG, DyLight® 633 Conjugate is a secondary antibody conjugated to DyLight® 633, which binds to all rabbit IgGs in immunological assays.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

CAS Number

9007-83-4

HS Code

38221900

Host

Goat

Reactivity

Rabbit IgG heavy and light chains (H&L)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified Rabbit IgG, whole molecule

Clonality

Polyclonal

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)

Field of Research

Anti-Rabbit

Dilution

1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)

Purity

Immunogen affinity purified goat IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Precautions

Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.Purity of this antibody is > 95% based on SDS-PAGE.

Additionnal Information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight® 633 (Ex = 638 nm; Em = 658 nm)

Storage Conditions

Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Prediction Reactivity

Rabbit IgG Heavy and Light chains (H&L)

Frequently Asked Questions

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