Goat anti-Rabbit IgG (H&L), DyLight® 633 conjugated
Product Specifications
Background
Goat anti-rabbit IgG, DyLight® 633 Conjugate is a secondary antibody conjugated to DyLight® 633, which binds to all rabbit IgGs in immunological assays.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
CAS Number
9007-83-4
HS Code
38221900
Host
Goat
Reactivity
Rabbit IgG heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified Rabbit IgG, whole molecule
Clonality
Polyclonal
Applications
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
Field of Research
Anti-Rabbit
Dilution
1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)
Purity
Immunogen affinity purified goat IgG.
Format
Lyophilized
Reconstitution
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Precautions
Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.Purity of this antibody is > 95% based on SDS-PAGE.
Additionnal Information
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight® 633 (Ex = 638 nm; Em = 658 nm)
Storage Conditions
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Prediction Reactivity
Rabbit IgG Heavy and Light chains (H&L)
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