Anti-H1 | Histone H1
Product Specifications
Background
Histone 1 (H1)is a protein located in nuclei, incorporated into chromatin.
Host
Rabbit
Reactivity
Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
native H1 protein purified fromNicotiana tabaccum(H1A, H1B H1C,D,E,F)
Clonality
Polyclonal
Applications
Chromatin Immunoprecipitation (ChIP), Western blot (WB), Immunocytochemistry (ICC) (ICC)
Dilution
1 : 100-1 : 500 (ICC), 1 : 5000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
15 | 17 kDa
References & Citations
Rutowiczet al. (2019). Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis. Genome Biol. 2019 Aug 7;20(1):157. doi: 10.1186/s13059-019-1767-3. (western blot, immunolocalization)Benoitet al. (2018). Replication-coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development. New Phytol. 2018 Jun 13. doi: 10.1111/nph.15248.Wollmannet al. (2017). The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana. Genome Biol. 2017 May 18;18(1):94. doi: 10.1186/s13059-017-1221-3.Sheand Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. | doi: 10.3389/fpls.2015.00294.Sheet al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc670031/datasheet/as11 1801.pdf
CAS Number
9007-83-4
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