Anti-H1 | Histone H1

Product Specifications

Background

Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Native H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C, D, E, F)

Clonality

Polyclonal

Applications

Chromatin Immunoprecipitation (ChIP), Western blot (WB), Immunocytochemistry (ICC) (ICC)

Field of Research

Nuclear signaling

Dilution

1 : 100-1 : 500 (ICC), 1 : 5000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

15 | 17 kDa

References & Citations

Rutowicz et al. (2019) . Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis. Genome Biol. 2019 Aug 7;20 (1) :157. doi: 10.1186/s13059-019-1767-3. (western blot, immunolocalization) Benoit et al. (2018) . Replication-coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development. New Phytol. 2018 Jun 13. doi: 10.1111/nph.15248.Wollmann et al. (2017) . The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana. Genome Biol. 2017 May 18;18 (1) :94. doi: 10.1186/s13059-017-1221-3.She and Baroux (2015) . Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. | doi: 10.3389/fpls.2015.00294.She et al. (2013) . Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140 (19) :4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.