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Anti-PsbA | D1 protein of PSII, N-terminal

Product Specifications

Background

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

4962477

UniProt

A4QJR4

Host

Rabbit

Reactivity

Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Chlorella vulgaris, Mesembryanthemum crystallinum, Synechococcus sp. PCC7942, sp. PCC7002, Synechocystis 6803 substrain PCC-M

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide derived from N-terminal of available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

PSII (Photosystem II)

Dilution

1 : 1000 - 1 : 10 000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

38 | 28-30 kDa

Precautions

This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel

Additionnal Information

Peptide target used for antibody production comes from Helix 1 of PSII, lumenal exposed loop. Antibodies are going to recognize the target in a wide range of species.This product can be sold containing ProClin if requested.

References & Citations

Li et al. (2025) . Salicylic acid and ROS signaling modulate hypocotyl elongation in darkness via NPR1 and EX1. Sci Adv. 2025 Oct 31;11 (44) :eadx4417. doi: 10.1126/sciadv.adx4417.Pilarska et al. (2025) . Salinity-induced changes in the PSII/LHCII phosphorylation and organization of the photosynthetic protein complexes in the halophyte Mesembryanthemum crystallinum L. Journal of Plant Physiology, 10 July 2025, 154567. Nagy et al. (2023) . Photoautotrophic and sustained H2 production by the pgr5 mutant of Chlamydomonas reinhardtii in simulated daily light conditions. International Journal of Hydrogen Energy Volume 53, 31 January 2024, Pages 760-769. Chen et al. (2021) Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis, Plant Science, Volume 307,2021,110902, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.110902. Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel) . 2020 May 14;10 (5) :E63. doi: 10.3390/life10050063..Górecka et al. (2019) . Photosystem II 22kDa protein level a prerequisite for excess light-inducible memory, cross-tolerance to UV-C, and regulation of electrical signalling. Plant Cell Environ. 2019 Nov 23. doi: 10.1111/pce.13686.Liu et al. (2018) . Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002. Plant Cell Physiol. 2018 Jul 1;59 (7) :1466-1482. doi: 10.1093/pcp/pcy080.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Algae (brown and red), Conifers, Cyanobacteria, Brassica napus, Diatoms, Glycine max, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us

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