Anti-ADGP | ADP-glucose pyrophosphorylase
Product Specifications
Background
ADP-glucose pyrophosphorylase (AGPase) catalyses the first committed step in the synthesis of transient starch in chloroplasts and storage starch in amyloplasts in plants (Tetlow et al., 2004) . AGPase in higher plants is composed of two distinct subunits encoded by separate genes, forming an L2S2 heterotetramer. The large subunits (L) are modulators of allosteric activity, while the small subunits (S) are the catalytic subunits (Kim et al., 2007) . Synonymes:ADP-glucose pyrophosphorylase, ADP-glucose synthase, AGPase B, Alpha-D-glucose-1-phosphate adenyl transferase
CAS Number
9007-83-4
HS Code
38221900
Gene ID
834883
UniProt
P55228 , P55229 , P55231
Host
Rabbit
Reactivity
Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Triticum aestivum, Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Part of Arabidopsis thaliana recombinant ADP-glucose pyrophosphorylase small subunit, TAIR: At5g48300, UniProt: P55228
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Carbohydrates
Dilution
1 : 1000-1 : 5000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
49.4 | 52 kDa
Precautions
Cross reactivity of this antibody to Rubisco has been excluded using 2D gel electrophoresis.Antigen used to elicit this antbody has very poor conservation in large subunit of ADP-glucose pyrophosphorylase.
References & Citations
Lim et al (2022) . Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13 (1) :652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Ma et al. (2021) A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals. Nat Genet. 2021 Apr 29. doi: 10.1038/s41588-021-00855-6. Epub ahead of print. PMID: 33927398.Chang et al. (2020) . Enhanced lipid productivity in AGP knockout marine microalga Tetraselmis sp. using a DNA-free CRISPR-Cas9 RNP method. Bioresource Technology.Volume 303, May 2020, 122932. Ancin et al. (2019) . NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves.Plants (Basel) . 2019 Nov 26;8 (12) . pii: E543. doi: 10.3390/plants8120543.Takahashi et al. (2019) . Glutelin subtype-dependent protein localization in rice grain evidenced by immunodetection analyses. Plant Mol Biol. 2019 Mar 25. doi: 10.1007/s11103-019-00855-5.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Chlamydiae, Chlamydomonas reinhardtii, Cyanobacteria, Beta vulgaris, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Planctomycetes, Proteobacteria, Solanum lycopersicum, Spirochetes, Verrucomicrobia, Vitis vinifera
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