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Anti-RGA | DELLA protein RGA

Product Specifications

Background

DELLA protein RGA is a putative transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. It is a member of VHIID/DELLA regulatory family and is the most sensitive to GA application, compare to other DELLA proteins.Involved in fruit and flower development. Synonymes: GAI-related sequence, GRAS family protein 10, AtGRAS-10, Repressor on the ga1-3 mutant, Restoration of growth on ammonia protein 1.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

814686

UniProt

Q9SLH3

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

Brassica napus, Populus sp., Rosa chinensis, Triticum aestivum

Immunogen

KLH-conjugated peptide chosen from RGA of Arabidopsis thaliana UniProt: Q9SLH3, TAIR: At2g01570

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Biosynthesis/regulation

Dilution

1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

64 | 64 kDa

Precautions

RGA protein is prone to degradation therefore caution has to be taken during protein extraction

References & Citations

Dong et al. (2021) . An HB40 - Jungbrunnen1 - GA 2-OXIDASE regulatory module for gibberellin homeostasis in ArabidopsisYan et al. (2020) . FKF1 F-box Protein Promotes Flowering in Part by Negatively Regulating DELLA Protein Stability Under Long-Day Photoperiod in Arabidopsis. J Integr Plant Biol . 2020 May 19. doi: 10.1111/jipb.12971.Ferrero et al. (2019) . Class I TCP transcription factors target the gibberellin biosynthesis gene GA20ox1 and the growth promoting genes HBI1 and PRE6 during thermomorphogenic growth in Arabidopsis. Plant Cell Physiol. 2019 Jul 11. pii: pcz137. doi: 10.1093/pcp/pcz137.Lorrai et al. (2018) . Abscisic acid inhibits hypocotyl elongation acting on gibberellins, DELLA proteins and auxin. AoB Plants. 2018 Oct 5;10 (5) :ply061. doi: 10.1093/aobpla/ply061.Chahtane et al. (2018) . The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis. Elife. 2018 Aug 28;7. pii: e37082. doi: 10.7554/eLife.37082.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Arabidopsis thaliana

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