Goat anti-Mouse IgG (heavy chain), TRITC conjugated

Goat anti-mouse IgG Υ is a Rhodamine (TRITC) conjugated secondary antibody which binds to mouse IgG (heavy chain) . Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase mouse IgG.

This antibody reacts with the heavy chains on mouse IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.