Goat anti-Rabbit IgG (H&L), TRITC conjugated, min, cross-reactivity to bovine, goat, human, mouse, rat IgG

Goat anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody, which binds to all rabbit IgGs in immunological assays. It has been specially adsorbed against bovine, goat, human, mouse and rat IgG. (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rabbit IgG.

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.