Donkey anti-Rat IgG (H&L), HRP conjugated
Product Specifications
Background
Donkey anti-rat IgG (H&L), is a secondary HRP (horse radish peroxidase) conjugated antibody which binds to rat IgGs in immunological assays.
Host
Donkey
Clonality
Polyclonal
Dilution
The optimal working dilution should be determined by the investigator
Purity
Immunogen affinity purified donkey IgG.
Format
Lyophilized
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
References & Citations
Buiet al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.
Storage Conditions
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Product Datasheet
https://cdn.gentaur.com/products/451/686bf4632da8eae897d65041/datasheet/as10 947.pdf
CAS Number
9007-83-4
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