Goat anti-Rabbit IgG Fc, HRP conjugated

Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.

This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.