What is the catalog number for Goat anti-Rabbit IgG (H&L), F (ab) '2 fragment, HRP conjugated?

The catalog number for Goat anti-Rabbit IgG (H&L), F (ab) '2 fragment, HRP conjugated is AS10 852.

What sizes are available for Goat anti-Rabbit IgG (H&L), F (ab) '2 fragment, HRP conjugated?

Goat anti-Rabbit IgG (H&L), F (ab) '2 fragment, HRP conjugated is available in the following size(s): 0.5 mg.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Goat anti-Rabbit IgG (H&L), F (ab) '2 fragment, HRP conjugated

Selected Variant

AS10 852

Size: 0.5 mg

Product Specifications

Background

Goat anti-rabbit IgG (H&L), F (ab) '2 fragment is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

CAS Number

9007-83-4

HS Code

38221900

Host

Goat

Reactivity

Rabbit IgG (H&L)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified Rabbit IgG (H&L)

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Field of Research

Anti-Rabbit

Dilution

The optimal working dilution should be determined by the investigator

Purity

Immunogen affinity purified goat IgG, F (ab) '2 fragment.

Format

Lyophilized

Reconstitution

For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

Precautions

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Additionnal Information

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0) HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10% (w/v) BSA, Protease/IgG free0.1% (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

References & Citations

Sun et al. (2025) .The plastid cysteine synthase complex regulates ABA biosynthesis and stomatal closure in Arabidopsis.Nat Commun. 2025 Oct 8;16 (1) :8960.doi: 10.1038/s41467-025-64705-3. Gong et al. (2024) . HYPK controls stability and catalytic activity of the N-terminal acetyltransferase A in Arabidopsis thaliana. Cell Rep. 2024 Feb 15;43 (2) :113768.doi: 10.1016/j.celrep.2024.113768. Linster et al. (2015) . Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.

Storage Conditions

Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Prediction Reactivity

Rabbit IgG (H&L)

Price

Documents

Product Datasheet

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Frequently Asked Questions

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