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Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated

Product Specifications

Background

Goat anti-rabbit IgG (H&L), F(ab)'2 fragmentis a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) inimmunological assays.

Host

Goat

Reactivity

Rabbit IgG (H&L)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified Rabbit IgG (H&L)

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Dilution

The optimal working dilution should be determined by the investigator

Purity

Immunogen affinity purified goat IgG, F(ab)'2 fragment.

Format

Lyophilized

Reconstitution

For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

Precautions

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

References & Citations

Gonget al. (2024).HYPK controls stability and catalytic activity of the N-terminal acetyltransferase A in Arabidopsis thaliana. Cell Rep. 2024 Feb 15;43(2):113768.doi: 10.1016/j.celrep.2024.113768.Linsteret al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.

Storage Conditions

Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Product Datasheet

https://cdn.gentaur.com/products/451/686bf4632da8eae897d65193/datasheet/as10 852.pdf

CAS Number

9007-83-4

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