Goat anti-Rabbit IgG (H&L), F (ab) '2 fragment, Unconjugated
Selected Variant
AS10 846
Size: 1 mg
Product Specifications
Background
Goat anti-rabbit IgG (H&L), F (ab) '2 fragment is a secondary antibody, which binds to rabbit IgG in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Goat
Reactivity
Rabbit IgG heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified Rabbit IgG, whole molecule
Clonality
Polyclonal
Applications
ELISA (ELISA)
Field of Research
Anti-Rabbit
Dilution
The optimal working dilution should be determined by the investigator
Purity
Immunogen affinity purified goat IgG, F (ab) '2 fragment.
Format
Lyophilized
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
Precautions
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Additionnal Information
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Purity is ≥ 90 % based on SDS-PAGE. May contain small amounts of intact IgG.
Storage Conditions
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Prediction Reactivity
Rabbit IgG Heavy and Light chains (H&L)
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