Donkey anti-Rabbit IgG (H&L), ALP conjugated
Product Specifications
Background
Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Donkey
Reactivity
Rabbit IgG (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified Rabbit IgG
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Anti-Rabbit
Dilution
1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), , 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Purity
Immunogen affinity purified donkey IgG.
Format
Liquid
Precautions
This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
Additionnal Information
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
References & Citations
Kay et al. (2014) . Elevations in Th2-initiating cytokines (IL-33, IL-25, TSLP) in lesional skin from Chronic Spontaneous ("Idiopathic") Urticaria. Br J Dermatol. 2014 Dec 18. doi: 10.1111/bjd.13621.
Storage Conditions
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Prediction Reactivity
Rabbit IgG (H&L)
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