Goat anti-Human IgG (H&L), F (ab) '2 fragment, HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum

Goat anti-human IgG (H&L), F (ab) '2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F (ab) '2 fragment in immunological assays.

This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10% (w/v) BSA, Protease/IgG free0.1% (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG

Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.