Goat anti-Human IgG (H&L), F (ab) '2 fragment, ALP conjugated

Goat anti-human IgG (H&L), F (ab) '2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.

This antibody reacts with the alpha chains on human IgG based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Purity is ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.