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Goat anti-Human IgG (H&L), HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum

Selected Variant

AS10 772

Size: 1 mg

Product Specifications

Background

Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.

CAS Number

9007-83-4

HS Code

38221900

Host

Goat

Reactivity

Human IgG (H&L)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified human IgG

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Dilution

The optimal working dilution should be determined by the investigator

Purity

Immunogen affinity purified goat IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

Precautions

This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.

Additionnal Information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

Storage Conditions

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Prediction Reactivity

Human IgG (H&L)

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