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Goat anti-Human IgG (H&L), ALP conjugated

Product Specifications

Background

Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.

CAS Number

9007-83-4

HS Code

38221900

Host

Goat

Reactivity

Human IgG (H&L)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified human IgG

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Dilution

1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Purity

Immunogen affinity purified goat IgG.

Format

Liquid

Precautions

This antibody reacts with alpha (heavy chains) on human IgG and all light chains on human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based in immunoelectrophoresis.

Additionnal Information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

Storage Conditions

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Prediction Reactivity

Human IgG (H&L)

Frequently Asked Questions

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