Goat anti-Human IgE heavy (epsilon chain), HRP conjugated
Product Specifications
Background
Goat anti-human IgE heavy (epsilon chain)is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) inimmunological assays.
Host
Goat
Reactivity
Human IgE heavy (epsilon chain)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified human IgE
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Dilution
The optimal working dilution should be determined by the investigator
Purity
Immunogen affinity purified goat IgG.
Format
Lyophilized
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
Precautions
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins
Storage Conditions
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Product Datasheet
https://cdn.gentaur.com/products/451/686bf4632da8eae897d65169/datasheet/as10 754.pdf
CAS Number
9007-83-4
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