Goat anti-Human IgE heavy (epsilon chain), ALP conjugated
Product Specifications
Background
Goat anti-human IgE (epsilon chain)is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE (epsilon chain) inimmunological assays.
Host
Goat
Reactivity
Human Human IgE (epsilon chain)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
purified human IgE
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Dilution
1 : 500-1 :2000 (ELISA) , 1 : 20 -1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (WB)
Purity
Immunogen affinity purified goat IgG.
Format
Liquid
Precautions
No reactivity is observed to light chains or non-IgE human serum proteins based in immunoelectrophoresis
Storage Conditions
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity, Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Product Datasheet
https://cdn.gentaur.com/products/451/686bf4632da8eae897d64ecd/datasheet/as10 750.pdf
CAS Number
9007-83-4
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