Anti-HTA9 | Probable histone H2A variant 3
Product Specifications
Background
Variant histone H2A may replace conventional H2A in a subset of nucleosomes. Synonymes: H2A.F/Z 3
Host
Rabbit
Reactivity
Arabidopsis thaliana
Not reactive in
Nicotiana tabacum, Zea mays
Immunogen
KLH-conjugated synthetic peptide derived fromArabidopsis thaliana HTA9 UniProt:Q9C944, TAIR:At1g52740
Clonality
Polyclonal
Applications
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
Dilution
6 µg (ChIP), 1: 100 (IP), 1 : 1000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
14.2 kDa (Arabidopsis thaliana)
References & Citations
Abelendaet al. (2022) High ambient temperature impacts on flowering time in Brassica napus through both H2A.Z-dependent and independent mechanisms [published online ahead of print, 2022 Dec 27]. Plant Cell Environ. 2022;10.1111/pce.14526. doi:10.1111/pce.14526Sun, Yin, Ma, et al. (2022) Feedback regulation of auxin signaling through the transcription of H2A.Z and deposition of H2A.Z to SMALL AUXIN UP RNAs in Arabidopsis. New Phytol. 2022;236(5):1721-1733. doi:10.1111/nph.18440Bieluszewskiet al. (2022) NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5. PMID: 35022409; PMCID: PMC8755797.Bieluszewskiet al. (2022) NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5. PMID: 35022409; PMCID: PMC8755797.Kralemannet al. (2020). Removal of H2Aub1 by ubiquitin-specific proteases 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis. Genome Biol. 2020 Jun 16;21(1):144.doi: 10.1186/s13059-020-02062-8.Gómez-Zambranoet al. (2019). The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity. Nat Commun. 2019 Jun 27;10(1):2828. doi: 10.1038/s41467-019-10773-1.Gómez-Zambranoet al. (2018). Arabidopsis SWC4 Binds DNA and Recruits the SWR1 Complex to Modulate Histone H2A.Z Deposition at Key Regulatory Genes. Mol Plant. 2018 Mar 29. pii: S1674-2052(18)30122-9. doi: 10.1016/j.molp.2018.03.014.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc67020a/datasheet/as10 718.pdf
CAS Number
9007-83-4
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