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Anti-HTA9 | Probable histone H2A variant 3

Product Specifications

Background

Variant histone H2A may replace conventional H2A in a subset of nucleosomes. Synonymes: H2A.F/Z 3

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

Nicotiana tabacum, Zea mays

Immunogen

KLH-conjugated synthetic peptide derived fromArabidopsis thaliana HTA9 UniProt:Q9C944, TAIR:At1g52740

Clonality

Polyclonal

Applications

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP),  Western blot (WB)

Dilution

6 µg (ChIP), 1: 100 (IP), 1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

14.2 kDa (Arabidopsis thaliana)

References & Citations

Abelendaet al. (2022) High ambient temperature impacts on flowering time in Brassica napus through both H2A.Z-dependent and independent mechanisms [published online ahead of print, 2022 Dec 27]. Plant Cell Environ. 2022;10.1111/pce.14526. doi:10.1111/pce.14526Sun, Yin, Ma, et al. (2022) Feedback regulation of auxin signaling through the transcription of H2A.Z and deposition of H2A.Z to SMALL AUXIN UP RNAs in Arabidopsis. New Phytol. 2022;236(5):1721-1733. doi:10.1111/nph.18440Bieluszewskiet al. (2022) NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5. PMID: 35022409; PMCID: PMC8755797.Bieluszewskiet al. (2022) NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5. PMID: 35022409; PMCID: PMC8755797.Kralemannet al. (2020). Removal of H2Aub1 by ubiquitin-specific proteases 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis. Genome Biol. 2020 Jun 16;21(1):144.doi: 10.1186/s13059-020-02062-8.Gómez-Zambranoet al. (2019). The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity. Nat Commun. 2019 Jun 27;10(1):2828. doi: 10.1038/s41467-019-10773-1.Gómez-Zambranoet al. (2018). Arabidopsis SWC4 Binds DNA and Recruits the SWR1 Complex to Modulate Histone H2A.Z Deposition at Key Regulatory Genes. Mol Plant. 2018 Mar 29. pii: S1674-2052(18)30122-9. doi: 10.1016/j.molp.2018.03.014.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70b994c49e6dc67020a/datasheet/as10 718.pdf

CAS Number

9007-83-4

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