Anti-FtsZ | Procaryotic cell division GTPase

Product Specifications

Background

FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

944786

UniProt

P0A9A6

Host

Rabbit

Reactivity

Candidatus Moranella endobia PCIT , Escherichia DH5a, Escherichia coli BW 25113, Shigella flexneri

Not reactive in

Alysiella filiformis, B. subtilis, Haloferax mediterranei (Listera sp., Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus (strain MRSA252), cyanobacteria

Immunogen

KLH-conjugated synthetic peptide derived from known bacterial sequences of FtsZ including E.coli UniProt: P0A9A6

Clonality

Polyclonal

Applications

Immunofluorescence (IF), Western blot (WB)

Field of Research

Bacteria

Dilution

1 : 100-1 : 200 (IF), 1 : 1000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

40 | 42 kDa

References & Citations

Pokhrel et al. (2025) . Uropathogenic Escherichia coli proliferate as a coccoid morphotype inside human host cells.PLoS Biol. 2025 Sep 3;23 (9) :e3003366. doi: 10.1371/journal.pbio.3003366. Chakraborty et al. (2024) . Dynamics of interdomain rotation facilitates FtsZ filament assembly. JBC, 7 May 2024, 107336. Vedyaykin et al. (2020) . SulA is able to block cell division in Escherichia coli by a mechanism different from sequestration. Biochem Biophys Res Commun . DOI: 10.1016/j.bbrc.2020.03.012 Ranjit et al. (2020) . Chlamydial MreB Directs Cell Division and Peptidoglycan Synthesis in Escherichia coli in the Absence of FtsZ Activity. mBio. 2020 Feb 18;11 (1) . pii: e03222-19. doi: 10.1128/mBio.03222-19. (Immunofluorescence) Sekar et al. (2018) . Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. Mol Syst Biol. 2018 Nov 5;14 (11) :e8623. doi: 10.15252/msb.20188623.Mückl et al. (2018) . Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach. PLoS One. 2018 Sep 11;13 (9) :e0198058. doi: 10.1371/journal.pone.0198058. eCollection 2018.Pende et al. (2014) . Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803.Söderström et al. (2014) . Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038, Xanthomonas oryzae