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Anti-H3 | Histone H3 (antigen affinity purified)

Product Specifications

Background

Histone 3 (H3)located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Host

Rabbit

Reactivity

Arabidopsis thaliana, Oryza sativa

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide derived from known H3 sequences, inludingArabidopsis thalianaH3.3P59169(At4g40030,At4g40040,At5g10980), H3.2P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2Q9FXI7(At1g19890)

Clonality

Polyclonal

Applications

ChIp-qPCR (ChIp-qPCR), Immunofluorescence (IF), Western blot (WB)

Dilution

2,5 µg/100 µg of chromatin (ChIp-qPCR), 1: 400 (IF), 1 : 5000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

15 | 17 kDa

Precautions

Protocol for isolation of cytosolic and nuclear fractions can be foundhere.Specific protol for ChIP can be found here:Salehet al. (2008).

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70b994c49e6dc670512/datasheet/as10 710a.pdf

CAS Number

9007-83-4

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