Anti-H3 | Histone H3 (antigen affinity purified)
Product Specifications
Background
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
830164
UniProt
P59169 , P59226 , Q9FXI7
Host
Rabbit
Reactivity
Arabidopsis thaliana, Oryza sativa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Clonality
Polyclonal
Applications
ChIp-qPCR (ChIp-qPCR), Immunofluorescence (IF), Western blot (WB)
Field of Research
Nuclear marker
Dilution
2,5 µg/100 µg of chromatin (ChIp-qPCR), 1: 400 (IF), 1 : 5000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
15 | 17 kDa
Precautions
Protocol for isolation of cytosolic and nuclear fractions can be foundhere.Specific protol for ChIP can be found here:Salehet al. (2008).
Additionnal Information
Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii
References & Citations
Storage Conditions
Prediction Reactivity
Documents
Frequently Asked Questions
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