Anti-3-nitroY | Nitrotyrosine

Product Specifications

Background

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Reactivity

Dog, Human, Mouse, Rat

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

BSA-conjugated 3- (4-Hydroxy-3-nitrophenyl acetamido) propionic acid

Clonality

Monoclonal

Applications

ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

Field of Research

Plant Oxidative Stress

Dilution

1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator

Purity

Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.

Format

Liquid

Precautions

The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivitywith non-nitrated tyrosine. Not species specific.0.7μg/mL was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.Antibody works paraffin-embedded sections.

Additionnal Information

1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol

References & Citations

Gow et al. (2004) .Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287 (2) : L262-8.Antibody used in immunohistochemistry:Pfister et al. (2002) . Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Girault et al. (2001) .Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11) : 1375-1387.

Storage Conditions

Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.