Anti-3-nitroY | Nitrotyrosine
Product Specifications
Background
Nitrotyrosineis a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Host
Mouse
Reactivity
Dog, Human, Mouse, Rat
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid
Clonality
Monoclonal
Applications
ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Dilution
1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
Purity
Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Format
Liquid
Precautions
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivitywith non-nitrated tyrosine. Not species specific.0.7μg/mL was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.Antibody works paraffin-embedded sections.
References & Citations
Gowet al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:Pfisteret al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Giraultet al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Storage Conditions
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc66fc16/datasheet/as10 706-100.pdf
CAS Number
9007-83-4
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