Anti-PsbA | D1 protein of PSII, DE-loop

Product Specifications

Background

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

CAS Number

9007-83-4

HS Code

38221900

Gene ID

844802

UniProt

P83755

Host

Rabbit

Reactivity

Arabidopsis thaliana, Chlamydomonas reinhardii, Hordeum vulgare, Medicago truncatula, Nannochloropsis oceanica strain IMET1, Neochloris oleoabundans UTEX 1185 (chlorophyta), Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide, amino acids 234-242 of Arabidopsis thaliana D1 protein UniProt: P83755, TAIR:AtCg00020

Clonality

Polyclonal

Applications

Blue Native PAGE (BN-PAGE), Western blot (WB)

Field of Research

PSII (Photosystem II)

Dilution

1 : 10 000, thylakoid fraction (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

38 | 28-30 kDa

Precautions

Antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoidsfor usage on total cell extracts the dilution needs to be determined experimentally

Additionnal Information

Antibody will detect 23 kDa N-terminal fragment

References & Citations

Woodford et al. (2025) . PROTON GRADIENT REGULATION 5 enables efficient C4 photosynthesis under fluctuating light. Plant Physiol. 2025 Sep 29:kiaf466.doi: 10.1093/plphys/kiaf466. Wang et al. (2025) . Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii. Nat Plants. 2025 Jul;11 (7) :1455-1469. doi: 10.1038/s41477-025-02036-3. Wójtowicz et al. (2025) . Shrink or expand? Just relax! Bidirectional grana structural dynamics as early light-induced regulator of photosynthesis. New Phytol . 2025 Jun;246 (6) :2580-2596. doi: 10.1111/nph.70175. Lu et al. (2021) . Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection. Nat Commun. 2021 Jan 29;12 (1) :679. doi: 10.1038/s41467-021-20967-1. PMID: 33514722; PMCID: PMC7846763. (blue-native PAGE) Mazur et al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187 (4) :2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Rantala et al. (2020) . PGR5 and NDH-1 systems do not function as protective electron acceptors but mitigate the consequences of PSI inhibition. Biochim Biophys Acta Bioenerg. 2020 Jan 11;1861 (3) :148154. doi: 10.1016/j.bbabio.2020.148154.Grieco et al. (2020) . Adjustment of photosynthetic activity to drought and fluctuating light in wheat. Plant Cell Environ. 2020 Mar 16. doi: 10.1111/pce.13756. Rantala and Tikkanen et al. (2018) . Phosphorylation?induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Cucumis sativus, Glycine max, Nannochloropsis sp., Oryza sativa, Populus balsamifera, Ricinus communis, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us