Anti-Clathrin heavy-chain 1,2, ALP-conjugated (40 µg)
Product Specifications
Background
Clathrinis a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Host
Rabbit
Reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
Not reactive in
Nicotiana benthamiana
Immunogen
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences includingArabidopsis thalianaclathrin heavy chain 1 UniProt:Q0WNJ6, TAIR:At3g11130,clathrin heavy chain 2 UniProt:Q0WLB5,TAIR:At3g08530
Clonality
Polyclonal
Applications
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Dilution
1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Format
Liquid
Molecular Weight
193 | 170 kDa (Arabidopsis thaliana)
Precautions
Please, note that fresh samples will provide better restuls (see image below)
References & Citations
Gaoet al. (2019). The Arabidopsis receptor kinase STRUBBELIG undergoes clathrin-dependent endocytosis. J Exp Bot. 2019 Apr 25. pii: erz190. doi: 10.1093/jxb/erz190.
Storage Conditions
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc670072/datasheet/as10 690-alp.pdf
CAS Number
9007-83-4
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