Anti-Clathrin heavy-chain 1,2, ALP-conjugated (40 µg)
Product Specifications
Background
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
820284
UniProt
Q0WNJ6
Host
Rabbit
Reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
Not reactive in
Nicotiana benthamiana
Immunogen
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5, TAIR:At3g08530
Clonality
Polyclonal
Applications
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Field of Research
Endomembrane system
Dilution
1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Format
Liquid
Molecular Weight
193 | 170 kDa (Arabidopsis thaliana)
Precautions
Please, note that fresh samples will provide better restuls (see image below)
References & Citations
Gao et al. (2019) . The Arabidopsis receptor kinase STRUBBELIG undergoes clathrin-dependent endocytosis. J Exp Bot. 2019 Apr 25. pii: erz190. doi: 10.1093/jxb/erz190.
Storage Conditions
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Prediction Reactivity
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera Species of your interest not listed? Contact us
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