Anti-HDEL | Endoplasmic reticulum retention signal (clone 2E7)
Product Specifications
Background
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein
Clonality
Monoclonal
Applications
Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Field of Research
Endomembrane system
Dilution
1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Format
Liquid
Molecular Weight
78 kDa
Precautions
Antibody in concentration 1 µg/mL was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
Additionnal Information
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
References & Citations
Luo et al. (2006) . GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26 (15) : 5688-5697.Napier et al. (1992) . Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
Storage Conditions
Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Higher plants
Isotype
IgG2B
Documents
Frequently Asked Questions
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