What is the catalog number for Anti-Tubulin beta chain?

The catalog number for Anti-Tubulin beta chain is AS10 681.

What sizes are available for Anti-Tubulin beta chain?

Anti-Tubulin beta chain is available in the following size(s): 100 µg.

Is Anti-Tubulin beta chain in stock?

Anti-Tubulin beta chain is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-Tubulin beta chain

Product Specifications

Background

Tubulin beta (TUB) together with alpha tubulin is making up microtubules.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

836390

UniProt

P29512 , P24636 , P29513 , P29514 , P29515 , P29516 , P29517

Host

Rabbit

Reactivity

Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa

Not reactive in

Allium sativum

Immunogen

KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512, beta-4 chain P24636 (At5g44340), beta-5 chain P29513 (At1g20010), beta-6 chain P29514 (At5g12250), beta-7 chain P29515 (At2g29550), beta-8 chain P29516 (At5g23860), beta-9 chain P29517 (At4g20890) .

Clonality

Polyclonal

Applications

Immunofluorescence (IF), Western blot (WB)

Field of Research

Cytoskeleton

Dilution

1 : 1000 (IF), 1 : 500 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

49 | 49 kDa (Arabidopsis thaliana)

Precautions

Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be foundhere.

Additionnal Information

Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below) . Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 µg/well and a dilution of 1: 500.

References & Citations

Gong et al. (2024) .HYPK controls stability and catalytic activity of the N-terminal acetyltransferase A in Arabidopsis thaliana. Cell Rep. 2024 Feb 15;43 (2) :113768.doi: 10.1016/j.celrep.2024.113768. Durian et al. (2019) . PROTEIN PHOSPHATASE 2A-B' gamma controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Wang et al. (2017) . The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40 (1) :56-68. doi: 10.1111/pce.12826.Heinnickel et al. (2016) . Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113 (10) :2774-9. doi: 10.1073/pnas.1524040113

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Brassica napus, Chlamydomonas reinhardii, Glycine max, Hordeum vulgare, Micromonoas pusilla, Nicotiana attenuata, Oryza sativa, Ostreococcus lucimarinus, Picea sitcHensis, Pisum sativum, Physcomitrium patens, Populus trichocarpa, Pythium ultimum, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Zea mays, Vigna radiata, Vitis viniferaSpecies of your interest not listed? Contact us