What is the catalog number for Anti-AGO5 | Argonaute 5?

The catalog number for Anti-AGO5 | Argonaute 5 is AS10 671.

What sizes are available for Anti-AGO5 | Argonaute 5?

Anti-AGO5 | Argonaute 5 is available in the following size(s): 50 µg.

Is Anti-AGO5 | Argonaute 5 in stock?

Anti-AGO5 | Argonaute 5 is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-AGO5 | Argonaute 5

Product Specifications

Background

AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) . This protein complex is responsible for the gene silencing (RNAi) . Probably involved in antiviral RNA silencing.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

817334

UniProt

Q9SJK3

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

Hordeum vulgare, Populus sp., Solanum lycopersicum, Zea mays

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana AGO5 protein sequence, UniProt: Q9SJK3, TAIR:AT2G27880

Clonality

Polyclonal

Applications

Immunofluorescebce (IF), Immunoprecipitation (IP), Western blot (WB)

Field of Research

Plant RNA

Dilution

1 : 100 (IF), 5 ug per gram floral tissue (IP), 1 : 1500 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

111 | 111 kDa

Precautions

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure

References & Citations

Martín-Merchán et al. (2024) . Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications. Nucleic Acids Res . 2024 May 20:gkae387.doi: 10.1093/nar/gkae387. Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.