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Goat anti-Rabbit IgG (H&L) HRP conjugated, min, cross-reactivity to bovine/Human/mouse IgG/serum

Selected Variant

AS10 668

Size: 1 mg

Product Specifications

Background

Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.

CAS Number

9007-83-4

HS Code

38221900

Host

Goat

Reactivity

Heavy chains of Rabbit IgG, Light chains on all Rabbit immunoglobulins

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified Rabbit IgG, whole molecule

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Field of Research

Anti-Rabbit

Dilution

1 : 500 -1 : 5000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 500 -1 : 5000 (WB)

Purity

Immunogen affinity purified goat IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Precautions

Based on immunoelectrophoresis no reactivity is observed to:non-immunoglobulin rabbit serum proteinsserum from bovine, human or mouseIgG from human or mouse

Additionnal Information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

References & Citations

Ghosh et al. 2023) .ApoE Isoforms Inhibit Amyloid Aggregation of Proinflammatory Protein S100A9.MDPI, Volume 25, Issue 410.3390/ijms25042114 Lacour et al. (2019) . Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis. Polar Biol (2019) . https://doi.org/10.1007/s00300-019-02507-2.

Storage Conditions

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Prediction Reactivity

Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins

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