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Goat anti-Rat IgG (H&L), ALP conjugated, min, reactivity to Human and mouse IgG, highly adsorbed against mouse IgG

Selected Variant

AS10 663

Size: 1 mg

Product Specifications

Background

Goat anti-rat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.

CAS Number

9007-83-4

HS Code

38221900

Host

Goat

Reactivity

Heavy chains on Rat IgG and with the light chains on all Rat immunoglobulins based on IEP

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified Rat IgG, whole molecule

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Field of Research

Anti-Rat

Dilution

1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Purity

Immunogen affinity purified goat IgG.

Format

Liquid

Precautions

This antibody is highly cross absorbed against mouse IgG, No reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis, No reactivity is observed to human or mouse IgG based on immunoelecrophoresis,

Additionnal Information

APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

References & Citations

Li et al. (2022), The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase. Limnol Oceanogr, 67: 1566-1576. https://doi.org/10.1002/lno.12151

Storage Conditions

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Prediction Reactivity

Heavy chains on Rat IgG and with the Light chains on all Rat immunoglobulins based on IEP

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