Anti-Enolase 2
Product Specifications
Background
Enolase (2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. Alternative name: Bifunctional enolase 2/transcriptional activator
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
Arabidopsis thaliana, Helianthus annuus
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Recombinant Arabidopsis thaliana enolase UniProt: P25696-1, TAIR: At2g36530
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Carbohydrates
Dilution
1 : 2000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 200 µl of sterile water
Molecular Weight
47.7 kDa (Arabidopsis thaliana)
Precautions
Antibody is specific for the ENO2 isoform (cytosolic and active isoform), see data below
References & Citations
Zhang et al. (2020) . A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun. 2020 Sep 9;11 (1) :4509.doi: 10.1038/s41467-020-18234-w. Zhang et al. (2018) . Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40 (9) :1834-1848. doi: 10.1111/pce.12989.Chen et al. (2009) System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolic regulation. Plant Physiol.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Brassica sp., Chlamydomonas reinhardii, Lycopersicum esculentum, Gossypium mexicanum, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Zea mays Species of your interest not listed? Contact us
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