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Anti-Enolase 2

Product Specifications

Background

Enolase (2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. Alternative name: Bifunctional enolase 2/transcriptional activator

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

Arabidopsis thaliana, Helianthus annuus

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Recombinant Arabidopsis thaliana enolase UniProt: P25696-1, TAIR: At2g36530

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Carbohydrates

Dilution

1 : 2000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Molecular Weight

47.7 kDa (Arabidopsis thaliana)

Precautions

Antibody is specific for the ENO2 isoform (cytosolic and active isoform), see data below

References & Citations

Zhang et al. (2020) . A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun. 2020 Sep 9;11 (1) :4509.doi: 10.1038/s41467-020-18234-w. Zhang et al. (2018) . Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40 (9) :1834-1848. doi: 10.1111/pce.12989.Chen et al. (2009) System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolic regulation. Plant Physiol.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Brassica sp., Chlamydomonas reinhardii, Lycopersicum esculentum, Gossypium mexicanum, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Zea mays Species of your interest not listed? Contact us

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