Donkey normal serum (2 ml)

Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.

Concentration: 60.0 mg/ml (Bradford, IgG standards) Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.Protein concentration is 60.0 mg/ml (Bradford, IgG standards) . Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.

Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC. For storage at 2-8°C, add a preservative to prevent growth of bacteria. Rehydrate with 2.2 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.