Sheep anti-Rabbit IgG (H&L), TRITC conjugated

Sheep anti-rabbit IgG (H&L)is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.