Rabbit anti-Rat IgG (H&L), HRP conjugated

Product Specifications

Background

Rabbit anti-rat IgG (H&L)is a secondary antibody conjugated to HRP, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).

Host

Rabbit

Immunogen

Purified rat IgG (H&L)

Clonality

Polyclonal

Dilution

This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator

Purity

Immunogen affinity purified rabbit IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

References & Citations

Quianet al. (2015). Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1α/CXCR4 Axis in Rats. Stem Cells International Volume 2015 (2015), Article ID 306836.

Storage Conditions

Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.