Rabbit anti-Human IgG (H&L), FITC conjugated

Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to FITC, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L) . Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0) . Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins.