Rabbit anti-Human IgG (H&L), Biotin conjugated

Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to biotin, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L) .

This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution (s) : 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0) . Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins