Goat anti-Rabbit IgG (H&L), ALP conjugated, min, cross-reactivity to human serum

Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to ALP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L) .

Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,50 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 30 mM triethanolamine, pH 7,2,5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth, Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins, Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.