Goat anti-biotin, HRP conjugated

Goat anti-biotin is a secondary antibody conjugated to HRP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.

Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml, Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,1 % (v/v) Kathon CG as preservative of bacterial growth

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.