Goat anti-Human kappa chain, FITC conjugated

Goat anti-human kappa (k) chain is a secondary antibody conjugated to FITC, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm, Emax = 518 nm.

(Flow cyt) The optimal working dilution should be determined by the investigator, Suggested starting dilution (s) : 1 : 20-1 : 2000 for most applications

Purity of this preparation is >95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0) . Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 1% (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.