Goat anti-Human IgM (µ chain), F (ab) '2 fragment, ALP conjugated, min, cross-reactivity to human IgG or IgA

Goat anti-human IgM (µ chain), ALP (alkaline phosphatase) conjugated, is a F (ab) '2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgG or IgA. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

This antibody reacts with:heavy (µ) chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to: non-immunoglobulin serum proteins human IgG or IgA.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1% (w/v) BSA, Protease/IgG free, 0.05 % (w/v) sodium azide as preservative.

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.