Chicken anti-Goat IgG (H&L), TRITC conjugated, min, cross-reactivity to human, mouse, rabbit IgG/serum

Chicken anti-goat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed againt human, mouse, rabbit IgG/serum and affinity purified using solid phase goat IgG.

Antibody reacts with heavy chains on goat IgG and light chains on all goat immunoglobulins.Minimum cross reactivity is observed to non-immunoglobulin goat serum proteins and human, mouse and rabbit IgG/serum based on immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.