Donkey anti-Goat IgG (H&L), ALP conjugated

Donkey anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.