Donkey anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to mouse IgG/serum
Product Specifications
Background
Donkey anti-rabbit IgG (H&L), HRP conjugatedis a secondary antibody which binds to all rabbit IgGs in immunolgical assays. Antibodies are affinity purified using solid phase rabbit IgG.
Host
Donkey
Clonality
Polyclonal
Dilution
1 : 500 -1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Purity
Immunogen affininity purified IgG.
Format
Lyophilized
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
Storage Conditions
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Product Datasheet
https://cdn.gentaur.com/products/451/686bf4632da8eae897d6502d/datasheet/as10 1014.pdf
CAS Number
9007-83-4
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