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Donkey anti-Sheep IgG (H&L), DyLight® 488 conjugated

Product Specifications

Background

Donkey anti-Sheep IgG (H&L), DyLight®488 Conjugatedis a secondary antibody conjugated toDyLight® 488, which binds to Sheep IgG (H&L) in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Host

Donkey

Immunogen

Purified Sheep IgG, whole molecule

Clonality

Polyclonal

Dilution

1 : 20-1 : 2000 for most applications

Purity

Immunogen affinity purified donkey IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

Precautions

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Storage Conditions

Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Product Datasheet

https://cdn.gentaur.com/products/451/686bf4632da8eae897d650a3/datasheet/as09 631.pdf

CAS Number

9007-83-4

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