Rabbit anti-Goat IgG (H&L), ALP conjugated
Product Specifications
Background
Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all goat immunoglobulins in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
Goat IgG heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified Goat IgG, whole molecule
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Anti-Goat
Dilution
1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Purity
Immunogen affinity purified rabbit IgG.
Format
Liquid
Precautions
No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Additionnal Information
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Storage Conditions
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Prediction Reactivity
Goat IgG Heavy and Light chains (H&L)
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